Nesterov Semen Valeryevich

Senior researcher

Biography Nesterov Semen Valeryevich

In 2008 he graduated from high school with a gold medal and entered the Faculty of General and Applied Physics (Department of Physics and Technology of Nanostructures) of the Moscow Institute of Physics and Technology (MIPT). Starting from the 4th year at the university (since 2011), he began to carry out research aimed at establishing the mechanisms of functioning of mitochondrial energy systems under the guidance of Professor L.S. Yaguzhinsky. In 2014, he graduated with honors from the MIPT with a degree in Biophysics, after which he started postgraduate education at the Department of Biophysics of MIPT. At the same time, he began his work at the Research Institute of Cytochemistry and Molecular Pharmacology, in the Department of Bioenegetics. In 2019, he defended a dissertation “Signals of low glutamate concentrations in mitochondria: detection of glutamate receptors and induction of ROS synthesis” and get a PhD degree in Biophysics at MIPT.
He is familiar with modern physicochemical research methods. He is a specialist in the functions and structure of mitochondria (the activity of mitochondrial respiratory chain complexes, the efficiency of ATP synthesis, the synthesis of reactive oxygen species, the analysis of electron microscopy results). He has experience working with purified mitochondria isolated from organs of model animals or cell lines. Used an enzyme immunoassay in his work, collaborated with specialists in electron microscopy, atomic force microscopy, nuclear magnetic resonance, neutron and x-ray scattering. He has experience in analyzing large volumes of scientific literature data. Masters computer processing and visualization of molecular structures.

The main results of scientific work:
The main studies were aimed at studying the mechanisms of regulation and functioning of the oxidative phosphorylation system of mitochondria. In the course of experimental work, in the mitochondria of the brain and heart cells the presence of classical signaling receptors was discovered, which, as previously thought, were included only in the work of the nervous system. The presence of the glutamate NMDA receptor in mitochondria was rigorously proven and the presence of gamma-aminobutyric acid receptor subunits was detected. These results formed the basis of the dissertation.
In parallel, experimental studies were conducted, their results made it possible to directionally change the structural and functional state of the entire multienzyme complex of the phosphorylating system, switching it into an operating mode corresponding to a supercomplex. In the course of the work, structural rearrangements in the protein-lipid membrane of the functioning mitochondria that accompany the transformation of the oxidative phosphorylation system to the state of the supercomplex were revealed and described.
In addition, a theoretical study was conducted aimed at studying specific metabolic changes during chronic diseases. A meta-analysis of biological experiments and clinical studies was carried out using diabetes mellitus as an example. During this analysis it was possible to identify the formation of a pathological autocatalytic process that occurs due to the mutual strengthening of biochemical processes with free radicals and physiological processes of blood microcirculation disturbance. The key role of mitochondrial dysfunction in maintaining pathological feedbacks was identified. The work carried out allowed to identify promising new areas for the treatment of complications of diabetes.
During the study of the cell signal systems controlled by amino acids, the concept of cyclic switching of metabolic modes of non-dividing cells (anabolism and catabolism) was developed and substantiated, and the key role of amino acids in this process was shown.